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Briefly, FeFAB cells are the modified CRFK cells that express β-galactosidase under the control of the FFV LTR, which is trans-activated by the FFV Tas protein in relation to。

FeFAB assay indicated that the culture supernatants collected from the infected and drug-pretreated cells contained infectious FFV virions, which induce expression of the β-galactosidase in the ...

The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

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